Promoting the accumulation of scopolamine and hyoscyamine in Hyoscyamus niger L. through EMS based mutagenesis
نویسندگان
چکیده
منابع مشابه
Determination of Hyoscyamine and Scopolamine in Four Hyoscyamus Species from Iran
The purpose of the present study was to determine the tropane alkaloid content of four Hyoscyamus species from Iran, i.e. H. pusillus L., H. niger L., H. reticulatus L. and H. kurdicus Bornm. Determination of alkaloids was performed by the HPLC method. Samples were extracted with chloroform-methanol-25% ammonium hydroxide 15:5:1 (V/V/V). HPLC separation was performed on Eurospher C18 reversed p...
متن کاملDetermination of Hyoscyamine and Scopolamine in Four Hyoscyamus Species from Iran
The purpose of the present study was to determine the tropane alkaloid content of four Hyoscyamus species from Iran, i.e. H. pusillus L., H. niger L., H. reticulatus L. and H. kurdicus Bornm. Determination of alkaloids was performed by the HPLC method. Samples were extracted with chloroform-methanol-25% ammonium hydroxide 15:5:1 (V/V/V). HPLC separation was performed on Eurospher C18 reversed p...
متن کاملQuantitative Analysis of l-Hyoscyamine in Hyoscyamus reticulatus L. by GC-MS
The routine quality control of a herbal drug preparation requires the identification and determination of the contents of the active ingredients in the product. Measurement alone is not sufficient; it is also necessary to know how objectively to evaluate the experimental results . There are 6 Hyoscyamus (Solanaceae) species growing in Turkey, none of which are endemic. Hyoscyamus (Solanaceae) s...
متن کاملOriginal Contribution IN VITRO HYOSCYAMINE AND SCOPOLAMINE PRODUCTION OF BLACK HENBANE (HYOSCYAMUS NIGER) FROM SHOOT TIP CULTURE UNDER VARIOUS PLANT GROWTH REGULATORS AND CULTURE MEDIA
Tropane alkaloids, especially hyoscyamine (HYO) and scopolamine (SCO), are widely used in medicine for their antispasmodic, anticholinergic, and sedative properties. This study was undertaken to investigate the root and shoot tropane alkaloids production from in vitro shoot tip (as explant) culture of Hyoscyamus niger under different plant growth regulators including auxins (IAA, 2, 4-D, IBA, N...
متن کاملEnhanced secretion of tropane alkaloids in Nicotiana tabacum hairy roots expressing heterologous hyoscyamine-6beta-hydroxylase.
Hyoscyamine-6beta-hydroxylase (H6H; EC 1.14.11.11) catalyses oxidative reactions in the biosynthetic pathway leading from hyoscyamine to the more pharmaceutically valuable tropane alkaloid scopolamine. The h6h gene encoding H6H from Hyoscyamus niger was introduced, under the control of the CaMV 35S promoter, into non-hyoscyamine-producing Nicotiana tabacum and hyoscyamine-producing Hyoscyamus m...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2020
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0231355